The protein content of three membrane protein preparations has been determined by the Lowry method with bovine serum albumin as a standard and also by quantitative amino acid analysis as an absolute method. The results differ considerably, the Lowry method giving 29-42% higher values. This implies t
โฆ LIBER โฆ
Internally standardized amino acid analysis for determining peptide/carrier protein coupling ratio
โ Scribed by Jonglin Tsao; Xi Lin; Henry Lackland; Guillermo Tous; Youling Wu; Stanley Stein
- Book ID
- 107718961
- Publisher
- Elsevier Science
- Year
- 1991
- Tongue
- English
- Weight
- 637 KB
- Volume
- 197
- Category
- Article
- ISSN
- 0003-2697
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A method for the determination of the composition of a conjugate between two different proteins by amino acid analysis followed by least-squares evaluation of the concentration ratio of the two components is presented. The method is based solely on calculations and avoids the use of labeled residues