Lowry protein determination on membrane preparations: Need for standardization by amino acid analysis
✍ Scribed by W.H.M. Peters; A.M.M. Fleuren-Jakobs; K.M.P. Kamps; J.J.H.H.M. de Pont; S.L. Bonting
- Publisher
- Elsevier Science
- Year
- 1982
- Tongue
- English
- Weight
- 324 KB
- Volume
- 124
- Category
- Article
- ISSN
- 0003-2697
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✦ Synopsis
The protein content of three membrane protein preparations has been determined by the Lowry method with bovine serum albumin as a standard and also by quantitative amino acid analysis as an absolute method. The results differ considerably, the Lowry method giving 29-42% higher values. This implies that many published data for such proteins, based on Lowry protein determinations with bovine serum albumin as the generally applied standard, are in error. Suggestions are made on how to standardize the Lowry method so that reliable values can be obtained for membrane protein.