Internal fragmentation of proteins in polyacrylamide matrices for microsequencing
โ Scribed by R. Kellner; T. Houthaeve; T. Kurzchalia; P. Dupree; K. Simons
- Book ID
- 105273993
- Publisher
- Springer
- Year
- 1992
- Tongue
- English
- Weight
- 110 KB
- Volume
- 11
- Category
- Article
- ISSN
- 1573-4943
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๐ SIMILAR VOLUMES
A simple method for the quantitation of proteins on SDS-polyacrylamide gels, with lysozyme as an internal standard, has been designed. Gels containing known weight ratios of standard proteins to lysozyme were electrophoresed, stained with Coomassie blue R250, and scanned at 550 nm. Peak areas corres
Disc electrophoresis has been widely used as .a procedure for separating proteins. In many instances the gels have been analyzed for radioactivity and for protein content. However, the only available method for determining protein content has been microdensitometry, which is complex and requires exp