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Strategies for internal amino acid sequence analysis of proteins separated by polyacrylamide gel electrophoresis

โœ Scribed by Larry D. Ward; Gavin E. Reid; Robert L. Moritz; Richard J. Simpson


Publisher
Elsevier Science
Year
1990
Tongue
English
Weight
901 KB
Volume
519
Category
Article
ISSN
1873-3778

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We examined the different steps necessary for the enzymatic digestion of proteins in the polyacrylamide matrix after gel electrophoresis. As a result, we developed an improved method for obtaining peptides for internal sequence analysis from 1-2 micrograms of in-gel-digested proteins. The long washi