𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Interaction between the peptide glutathione and linoleic acid hydroperoxide

✍ Scribed by Zamora, Rosario ;Millán, F. ;Hidalgo, F. J. ;Alaiz, M. ;Maza, Maria P. ;Olías, J. M. ;Vioque, E.

Publisher
John Wiley and Sons
Year
1989
Tongue
English
Weight
405 KB
Volume
33
Category
Article
ISSN
0027-769X

No coin nor oath required. For personal study only.

✦ Synopsis

Incubation experiments in order to study the influence of some parameters in the fluorescence products formation have been carried out. It has been observed a greater efficiency with the relation linoleic acid hydroperoxide:glutathione, 2: 1, and a temperature of 37 "C. The complex formed working with labelled linoleic acid has been separated in two main fractions. The first one (without radioactivity) is fluorescent with excitation and emission maxima at 350 nm and 440 nm, respectively. It has been confirmed that this fraction consists of a complex of glutathione and short chain aldehydes. The fluorescence of the complex did not decrease by treatment with NaBH, or with pH. The NH, and SH groups take part in the lipid-peptide complex formation. The second, highly radioactive fraction includes free hydroperoxides and short chain hydroxy fatty acids.

'

Acknowledgements

Assistance in experiments and analyses was given by Mr. J. L. NAVARRO.

📜 SIMILAR VOLUMES

Interaction of the mycotoxin penicillic
Interaction of the mycotoxin penicillic acid with glutathione and rat liver glutathione S-transferases
✍ Paul J. Dierickx; Jacques O. Beer 📂 Article 📅 1984 🏛 Springer Netherlands 🌐 English ⚖ 345 KB

The in vitro interaction of the mycotoxin penicillic acid (PA) with rat liver glutathione S-transferase (GST) was studied using reduced glutathione and 1-chloro-2,4-dinitrobenzene as substrates. The inhibition of the GST activity by PA in crude extracts was dose dependent. Each of the different GST

Interactions between proteins, peptides
Interactions between proteins, peptides and amino acids. New advances 1986–1989
✍ Cserháti, T. ;Szögyi, M. 📂 Article 📅 1990 🏛 John Wiley and Sons 🌐 English ⚖ 557 KB

The paperdealswith therecent achievementsin thestudy ofthevariousinteractions between proteins. peptides and amino acids. The interactions are classified according to the hydrophilic, hydrophobic or mixed character of the interactive forces. The effect of the interaction on protein (peptide) associa