The primary reaction between the cysteine residues of histone H3 and dithiobis-(nitrobenzoic acid) may be succeeded by thiol-disulfide interchange steps leading to intraor intermolecularly crosslinked H3 molecules. A chromatographic assay is applied to detect consecutive reactions of this type and i
INTER AND INTRAMOLECULAR INTERACTIONS OF PORPHYRINS WITH 5,5′-DITHIOBIS(2-NITROBENZOIC ACID)
✍ Scribed by Francis D'Souza; V. Krishnan
- Book ID
- 114896202
- Publisher
- John Wiley and Sons
- Year
- 1992
- Tongue
- English
- Weight
- 955 KB
- Volume
- 56
- Category
- Article
- ISSN
- 0031-8655
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Polarography of 5,5'-dithio bis-(2 nitrobenzoic acid) in the pH range 2-12, in Britton-Robinson buffer at constant ionic strength (0 .5 M) has been carried out. Four cathodic waves (A, B, C and D) and an anodic wave have been obtained . Wave A is produced by a reduction of pre-protonated nitro group
Received March gth, rg7o \_/.ClJb'Ok?&O&!., \_'jO (1970) 150-151 CHROM. 4738 Chromatographic detection of thiols, disulfides, and thioesters with 5,5'-dithiobis(2-nitrobenzoic acid)
Hydrogen sulfide was determined with N-ethylmaleimide (NEM), p-chloromercuribenzoate (PCMB), and 5,5'-dithiobiQ2nitrobenzoic acid) (DTNB). One mole of hydrogen sulfide reacted with two moles of NEM or PCMB. It reacted with only one mole of DTNB, but produced two moles of the thiol anion, 5thio-2nitr