Determination of hydrogen sulfide with 5,5′-dithiobis-(2-nitrobenzoic acid), N-ethylmaleimide, and parachloromercuribenzoate

✍ Scribed by Aws S. Nashef; David T. Osuga; Robert E. Feeney

• Publisher: Elsevier Science
• Year: 1977
• Tongue: English
• Weight: 660 KB
• Volume: 79
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(77)90413-4

✦ Synopsis

Hydrogen sulfide was determined with N-ethylmaleimide (NEM), p-chloromercuribenzoate (PCMB), and 5,5'-dithiobiQ2nitrobenzoic acid) (DTNB). One mole of hydrogen sulfide reacted with two moles of NEM or PCMB. It reacted with only one mole of DTNB, but produced two moles of the thiol anion, 5thio-2nitrobenzoate, and one mole of free sulfur. Concentrations of hydrogen sulfide down to 3.7 x 10eB~ (0.13 &ml) could be easily determined with DTNB. In the presence of proteins containing different amounts of sulfhydryls reacting with DTNB, 78 to 94% of the added hydrogen sulfide could be recovered.