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Inter- and intramolecular crosslinks in histone H3 induced by 5,5′-dithiobis(2-nitrobenzoic acid)

✍ Scribed by Jürgen Bode

Publisher
Elsevier Science
Year
1979
Tongue
English
Weight
335 KB
Volume
94
Category
Article
ISSN
0003-2697

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✦ Synopsis

The primary reaction between the cysteine residues of histone H3 and dithiobis-(nitrobenzoic acid) may be succeeded by thiol-disulfide interchange steps leading to intraor intermolecularly crosslinked H3 molecules. A chromatographic assay is applied to detect consecutive reactions of this type and is used to derive conditions by which they are promoted or suppressed. Elimination of the secondary interchange is shown to be essential if the kinetics of the reaction is to be interpreted.

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