INK4a-ARF mutations in skin carcinomas from UV irradiated hairless mice

✍ Scribed by N. Soufir; S. Queille; K. Mollier; E. Roux; A. Sarasin; F.R. de Gruijl; A.M. Fourtanier; L. Daya-Grosjean; N. Basset-Seguin

• Book ID: 102500780
• Publisher: John Wiley and Sons
• Year: 2004
• Tongue: English
• Weight: 68 KB
• Volume: 39
• Category: Article
• ISSN: 0899-1987
• DOI: 10.1002/mc.20004

✦ Synopsis

Abstract

To characterize further the role of the INK4a‐ARF locus in the multistep process of skin carcinogenesis, we performed a mutational analysis of this locus in skin lesions from hairless mice either irradiated with UVB alone or with a solar simulator delivering UVA + B. INK4a‐ARF mutations were present in five of 57 squamous cell carcinomas (9%), but no mutation was detected in precancerous lesions. All mutations were C:G > T:A transitions located at dipyrimidic sites, the hallmark of UVB mutagenesis. Three mutations affected only the p19^ARF^ reading frame, whereas two mutations affected only the p16^INK4a^ transcript. This study demonstrates for the first time UV‐induced mutations of INK4a‐ARF that occur in a small percentage in late stages skin tumors. © 2004 Wiley‐Liss, Inc.