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Initiation reactions in the mRNA-dependent reticulocyte lysate

โœ Scribed by John E. Kay; C.Robin Benzie


Book ID
119119041
Publisher
Elsevier Science
Year
1982
Tongue
English
Weight
269 KB
Volume
698
Category
Article
ISSN
0167-4781

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๐Ÿ“œ SIMILAR VOLUMES


An Efficient mRNA-Dependent Translation
โœ Hugh R. B. PELHAM; Richard J. JACKSON ๐Ÿ“‚ Article ๐Ÿ“… 1976 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 933 KB

A simple method is described for converting a standard rabbit reticulocyte cellโ€free extract (lysate) into an mRNAโ€dependent protein synthesis system. The lysate is preincubated with CaCl~2~ and micrococcal nuclease, and then excess ethyleneglycolโ€bis(2โ€aminoethylether)โ€N,Nโ€ฒโ€tetraacetic acid is adde