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An Efficient mRNA-Dependent Translation System from Reticulocyte Lysates

โœ Scribed by Hugh R. B. PELHAM; Richard J. JACKSON


Book ID
115115520
Publisher
John Wiley and Sons
Year
1976
Tongue
English
Weight
933 KB
Volume
67
Category
Article
ISSN
1432-1327

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An Efficient mRNA-Dependent Translation
โœ Hugh R. B. PELHAM; Richard J. JACKSON ๐Ÿ“‚ Article ๐Ÿ“… 1976 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 933 KB

A simple method is described for converting a standard rabbit reticulocyte cellโ€free extract (lysate) into an mRNAโ€dependent protein synthesis system. The lysate is preincubated with CaCl~2~ and micrococcal nuclease, and then excess ethyleneglycolโ€bis(2โ€aminoethylether)โ€N,Nโ€ฒโ€tetraacetic acid is adde