An Efficient mRNA-Dependent Translation
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Hugh R. B. PELHAM; Richard J. JACKSON
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Article
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1976
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John Wiley and Sons
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English
โ 933 KB
A simple method is described for converting a standard rabbit reticulocyte cellโfree extract (lysate) into an mRNAโdependent protein synthesis system. The lysate is preincubated with CaCl~2~ and micrococcal nuclease, and then excess ethyleneglycolโbis(2โaminoethylether)โN,Nโฒโtetraacetic acid is adde