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An mRNA-dependent in vitro translation system from Trypanosoma brucei

โœ Scribed by Silvia N.J. Moreno; Hon S. Ip; George A.M. Cross

Book ID
113260808
Publisher
Elsevier Science
Year
1991
Tongue
English
Weight
851 KB
Volume
46
Category
Article
ISSN
0166-6851

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A simple method is described for converting a standard rabbit reticulocyte cellโ€free extract (lysate) into an mRNAโ€dependent protein synthesis system. The lysate is preincubated with CaCl~2~ and micrococcal nuclease, and then excess ethyleneglycolโ€bis(2โ€aminoethylether)โ€N,Nโ€ฒโ€tetraacetic acid is adde