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Poly(A) dependent translation in rabbit reticulocyte lysate

✍ Scribed by M. Wakiyama; T. Futami; K. Miura


Book ID
117457122
Publisher
Elsevier Science
Year
1997
Tongue
English
Weight
911 KB
Volume
79
Category
Article
ISSN
0300-9084

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An Efficient mRNA-Dependent Translation
✍ Hugh R. B. PELHAM; Richard J. JACKSON 📂 Article 📅 1976 🏛 John Wiley and Sons 🌐 English ⚖ 933 KB

A simple method is described for converting a standard rabbit reticulocyte cell‐free extract (lysate) into an mRNA‐dependent protein synthesis system. The lysate is preincubated with CaCl~2~ and micrococcal nuclease, and then excess ethyleneglycol‐bis(2‐aminoethylether)‐N,N′‐tetraacetic acid is adde