Initiation of DNA Synthesis in Cell Cultures by Colcemid
β Scribed by J. M. Vasiliev, I. M. Gelfand and V. I. Guelstein
- Book ID
- 123650179
- Publisher
- National Academy of Sciences
- Year
- 1971
- Tongue
- English
- Weight
- 463 KB
- Volume
- 68
- Category
- Article
- ISSN
- 0027-8424
- DOI
- 10.2307/60628
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Myocardial cells cultured from ventricles of 4-day embryonic chick hearts were labeled with H3-thymidine for the radioautographic study of DNA synthesis and nuclear cycling. After the myoblasts had been in culture for 48 hours and had begun to grow out and contract, the cultures were exposed to H3-t
Chinese hamster fibroblasts in monolayer cultures were synchronized by accumulating mitotic cells in the presence of Colcemid, removing the mitotic cells with a brief trypsin treatment, and growing them in medium lacking Colcemid. Such cultures grew normally and exhibited no significant deviations f