Synchronized mammalian cell cultures. I. Cell replication cycle and macromolecular synthesis following brief colcemid arrest of mitosis
✍ Scribed by Elton Stubblefield; Robert Klevecz; Larry Deaven
- Publisher
- John Wiley and Sons
- Year
- 1967
- Tongue
- English
- Weight
- 767 KB
- Volume
- 69
- Category
- Article
- ISSN
- 0021-9541
No coin nor oath required. For personal study only.
✦ Synopsis
Chinese hamster fibroblasts in monolayer cultures were synchronized by accumulating mitotic cells in the presence of Colcemid, removing the mitotic cells with a brief trypsin treatment, and growing them in medium lacking Colcemid. Such cultures grew normally and exhibited no significant deviations from control cultures in their mitotic interval, generation time, DNA synthesis kinetics, or proliferative capacity.
The macromolecular composition of 1W mitotic cells was chemically determined to be: DNA, 15 fig; RNA, 28 fig; and protein, 190 pg. In stock cultures, the corresponding values were about 60% to 70% of those for mitotic cells.