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DNA synthesis by cultured myocardial cells

✍ Scribed by Rumery, Ruth E. ;Rieke, William O.

Publisher
John Wiley and Sons
Year
1967
Tongue
English
Weight
465 KB
Volume
158
Category
Article
ISSN
0003-276X

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✦ Synopsis

Myocardial cells cultured from ventricles of 4-day embryonic chick hearts were labeled with H3-thymidine for the radioautographic study of DNA synthesis and nuclear cycling. After the myoblasts had been in culture for 48 hours and had begun to grow out and contract, the cultures were exposed to H3-thymidine, 1 pc/ml of Hank's balanced salt solution, for one hour. Pairs of cultures were fixed in methyl alcohol for analysis every two hours over a subsequent period of 60 hours. The total

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