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Inhibition of uridine-cytidine kinase by 5-azacytidine 5′-triphosphate

✍ Scribed by Lee, Thomas T. ;Momparler, Richard L.

Publisher
John Wiley and Sons
Year
1976
Tongue
English
Weight
299 KB
Volume
2
Category
Article
ISSN
0098-1532

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✦ Synopsis

Abstract

5‐Azacytidine 5′‐triphosphate (5‐aza‐CTP) inhibited the phosphorylation of uridine, cytidine, and 5‐azacytidine (5‐aza‐C) in a reaction catalyzed by uridine‐cytidine kinase. The inhibition appeared to be competitive with respect to the adenosine 5′‐triphosphate and noncompetitive with respect to nucleoside substrates. 5‐aza‐CTP was a potent inhibitor of 5‐aza‐C phosphorylation but a weak inhibitor of uridine and cytidine phosphorylation. These results suggest that the feedback inhibition of uridine‐cytidine kinase by 5‐aza‐CTP may limit the amount of intracellular nucleotide analog formed in drug‐treated cells.

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