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Preparative-enzymatic formation of cytidine 5′-monophosphosialate by integrated cytidine 5′-triphosphate regeneration

✍ Scribed by Thiem, Joachim ;Stangier, Peter

Publisher
John Wiley and Sons
Year
1990
Tongue
English
Weight
458 KB
Volume
1990
Category
Article
ISSN
0947-3440

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✦ Synopsis

Abstract

The preparative formation of cytidine 5′‐triphosphate by an enzymatic process with adenylate kinase and pyruvate kinase is described. The enzymes may either be used free, in the “MEEC” technique, or immobilized on a synthetic copolymer of vinyl acetate and N,N′‐divinylethyleneurea (“VA‐Epoxy”). Cytidine 5′‐monophosphosialate synthase (preferably isolated from calf brain) and inorganic pyrophosphatase were used to prepare the activated neuraminic acid cytidine 5′‐monophosphosialate (CMP‐Neu5Ac) from CTP and neuraminic acid in good yield. Finally, both processes could be integrated and led to an in situ approach which, owing to moderate yields, requires further development.

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