Inhibition of gaba binding by pyridoxal and pyridoxal phosphate
β Scribed by Manuchair Ebadi; Borpit Klangkalya; Jean D. Deupree
- Publisher
- Elsevier Science
- Year
- 1980
- Tongue
- English
- Weight
- 671 KB
- Volume
- 11
- Category
- Article
- ISSN
- 0020-711X
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A new and very sensitive fiuorometric method for the determination of pyridoxal and pyridoxal 5'-phosphate is reported. The specificity is based on the reductive amination of pyridoxal and its Y-phosphate with methyl anthranilate and sodium cyanoborohydride at pH 4.5 to 5.0. Separation of the highly
Simultaneous and direct assays of pyridoxal, pyridoxal-5'-phosphate, and pyridoxic acid in human serum are described. The method applied is based on the reaction of these compounds with beryllium in an ammoniacal medium to yield highly fluorescent derivatives. Overlapping of conventional fluorescenc
## Abstract Schiff base formation by condensation of pyridoxal phosphate or pyridoxal with octopamine in aqueous or methanolic medium was investigated by ^13^C NMR. Enolimine forms are favoured in methanolic solution, whereas in aqueous solution the predominant ketoenamine forms show a hydrogen bon