Inhibition of DNA synthesis by methylglyoxal bis(guanylhydrazone) during lymphocyte transformation

✍ Scribed by Shuzo Otani; Yasuhiro Mizoguchi; Isao Matsui; Seiji Morisawa

• Publisher: Springer
• Year: 1974
• Tongue: English
• Weight: 290 KB
• Volume: 1
• Category: Article
• ISSN: 0301-4851
• DOI: 10.1007/bf00360667

✦ Synopsis

The phytohemagglutinin induced DNA synthesis in guinea pig lymph node cells was inhibited remarkably by methylglyoxal bis(guanylhydrazone). This inhibitory effect was dependent on the time of its addition to the lymph node cell culture after stimulation with phytohemagglutinin. If methylglyoxal bis (guanylhydrazone) was added 48 hr after the stimulation, no inhibition of DNA synthesis was observed. Exogenous spermidine added at an early time of cell culture reversed the inhibitory effect of methylglyoxal bis (guanylhydrazone). However, no reversion occurred when spermidine was added at a late time of the cell culture.

ABBREVIATIONS. PHA, phytohemagglutinin; MGBG, methylglyoxal bis(guanylhydrazone).