## Abstract A quantitative doseβresponse study was made of the inhibition by actinomycin D of the synthesis of various RNA species in cultured L cells. After a suitable preincubation with various concentrations of actinomycin, synthesis was measured by a brief incorporation of radioactive uridine.
Inhibition of Adrenocortical Responsiveness to ACTH by Actinomycin D in vivo
β Scribed by VERNIKOS-DANELLIS, JOAN; HALL, MADELINE
- Book ID
- 109645453
- Publisher
- Nature Publishing Group
- Year
- 1965
- Tongue
- English
- Weight
- 116 KB
- Volume
- 207
- Category
- Article
- ISSN
- 0028-0836
- DOI
- 10.1038/207766a0
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Actinomycin D (0.008 pg/gm of body weight) injected intraperitoneally every two hours, produced a prompt 50% inhibition of RNA synthesis in the jejunum of mice, and a delayed inhibition of DNA synthesis, that reached its maximum inhibition (68% of control values) 4.5 hours after the first injection