𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Infrequent loss of heterozygosity and mutation of the p53 gene in immortal and transformed mouse embryo fibroblasts

✍ Scribed by Masayuki Tokumitsu; Takayuki Kadohama; Katsuhiro Ogawa

Publisher
John Wiley and Sons
Year
1994
Tongue
English
Weight
557 KB
Volume
10
Category
Article
ISSN
0899-1987

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Some of the progeny of isolated mouse embryo fibroblasts acquire the ability to grow indefinitely during cultivation, presumably through some mutational events. The relevance of p53 mutations and loss of heterozygosity to the mechanism of such immortal growth capability remains controversial. Since four bases in intron 1 of the p53 gene in C3H/HeJ mice are replaced by 13 different bases in DBA/2J mice, it is possible to distinguish maternal and paternal p53 alleles in the cells of F1 hybrids of these strains (C3D2F1) by electrophoresis of polymerase chain reaction fragments including the region. We established 23 spontaneously immortalized fibroblast cell lines from C3D2F1 mouse embryos and 29 transformed cell lines induced from one of the immortal cell lines, either by treatment with chemical carcinogens or by transfection with the c‐Ha‐ras gene. Of these 52 cell lines, only one, derived from fibroblasts unpassaged for 4 mo, showed p53 gene loss of heterozygosity and a structural alteration in the remaining allele. Our results demonstrated that p53 mutations are not a strict requirement for immortalization and transformation of mouse embryo fibroblasts in Vitro. Β© 1994 Wiley‐Liss, Inc.

πŸ“œ SIMILAR VOLUMES

Transformation of normal human fibroblas
Transformation of normal human fibroblasts into immortalized cells with the mutant p53 gene and X-rays
✍ Kazuo Fushimi; Mikio Iijima; Chong Gao; Tadashi Kondo; Toshiya Tsjui; Tomoko Has πŸ“‚ Article πŸ“… 1997 πŸ› John Wiley and Sons 🌐 French βš– 255 KB πŸ‘ 2 views

In vitro cell transformation is a valuable approach for studying the mechanisms of multistep carcinogenesis of human cells. Since immortalization is an essential step for in vitro neoplastic transformation of human cells, this study addresses the question of whether mutant p53 contributes to the imm

Mutation, loss of heterozygosity, and re
Mutation, loss of heterozygosity, and recombination of the p53 gene in mouse forestomach tumors induced by 2-amino-3,4-dimethylimidazo[4,5-f]quinoline
✍ Toshikazu Ushijima; Hiroshi Makino; Hideo Okonogi; Yoko Hosoya; Takashi Sugimura πŸ“‚ Article πŸ“… 1995 πŸ› John Wiley and Sons 🌐 English βš– 573 KB

## Abstract 2‐Amino‐3,4‐dimethylimidazo[4,5‐__f__]quinoline (MelQ), a food mutagen, induces forestomach tumors in CDF~1~ mice. We established a polymerase chain reaction (PCR)–single‐strand conformation polymorphism (SSCP) analysis system to detect mutations in the mouse __p53__ gene exons 2–10, wh

Infrequent mutations of the TP53 gene an
Infrequent mutations of the TP53 gene and no amplification of the MDM2 gene in hepatoblastomas
✍ Hiroaki Ohnishi; Machiko Kawamura; Ryoji Hanada; Yasuhiko Kaneko; Yuko Tsunoda; πŸ“‚ Article πŸ“… 1996 πŸ› John Wiley and Sons 🌐 English βš– 363 KB πŸ‘ 2 views

We have investigated the mutation of the TP53 gene in hepatoblastomas (HBLs) by using polymerase chain reaction-single strand conformation polymorphism and direct sequencing in 38 HBL tumor samples and in two HBL cell lines. We detected the TP53 gene mutation in an anaplastic hepatoblastoma cell lin