Infrequent mutations of the TP53 gene and no amplification of the MDM2 gene in hepatoblastomas

✍ Scribed by Hiroaki Ohnishi; Machiko Kawamura; Ryoji Hanada; Yasuhiko Kaneko; Yuko Tsunoda; Teruaki Hongo; Fumio Bessho; Kinji Yokomori; Yasuhide Hayashi

• Publisher: John Wiley and Sons
• Year: 1996
• Tongue: English
• Weight: 363 KB
• Volume: 15
• Category: Article
• ISSN: 1045-2257
• DOI: 10.1002/(sici)1098-2264(199603)15:3<187::aid-gcc8>3.0.co;2-z

✦ Synopsis

We have investigated the mutation of the TP53 gene in hepatoblastomas (HBLs) by using polymerase chain reaction-single strand conformation polymorphism and direct sequencing in 38 HBL tumor samples and in two HBL cell lines. We detected the TP53 gene mutation in an anaplastic hepatoblastoma cell line, but no aberration of the TP53 gene (exons 5-9) was found in tumor samples and in the other HBL cell line. The mutation of the cell line was a missense mutation from GAC (asparagine) to CAC (histidine) at codon 28 I, which was different from the G-to-T transversion of codon 249 that is frequently found in adult hepatocellular carcinomas (HCCs). In addition, we performed Southern blot analysis of the MDMZ gene, but we did not find MDMZ gene amplification in I9 cases tested. Our results suggest that, in contrast to the findings in HCCs in adults, TP53 gene aberrations are not involved in the development or progression of HBLs in children.