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Influence of the modification procedure of support materials on the microenvironment of immobilized α-chymotrypsin

✍ Scribed by M.T. Martín; J.V. Sinisterra; A. Heras

Publisher
Elsevier Science
Year
1993
Weight
688 KB
Volume
80
Category
Article
ISSN
0304-5102

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✍ Wai-Keung Chui; Irving W. Wainer 📂 Article 📅 1992 🏛 Elsevier Science 🌐 English ⚖ 939 KB

Immobilized artificial membrane (IAM) HPLC supports have been used to immobilize the enzymes alpha-chymotrypsin and trypsin. The enzymes were trapped in hydrophobic cavities on the support and were not covalently attached to the IAM surface. The resulting IAM-enzyme supports retained the hydrolytic