Influence of mitoxantrone on nucleic acid synthesis on the T-47D breast tumor cell line

✍ Scribed by Ahmad R. Safa; Nasser Chegini; Michael T. Tseng

• Publisher: John Wiley and Sons
• Year: 1983
• Tongue: English
• Weight: 800 KB
• Volume: 22
• Category: Article
• ISSN: 0730-2312
• DOI: 10.1002/jcb.240220205

✦ Synopsis

Mitoxantrone exerts growth inhibitory effects, suppresses [3H]-thymidine as well as PHI-uridine incorporation, and induces ultrastructural alterations in T47D human breast tumor cells. At low concentration (10p9M) the drug induced little effect on cell proliferation; cell rowth kinetics were inhibited at a concentration concentrations tested and M), revealing a potent effect on metabolic activity of the cultured cells. The sharpest decline in DNA and RNA synthesis occurred within the first 2 hr of drug treatment. Serial ultrastructural examinations indicated definitive alterations in chromatin structure, disintegration of nucleolar components as early as 2 hr after drug treatment, and complete segregation of nucleolar components following 8-hr exposure to concentrations of the drug between lop5 and lop7 M. A distinct increase in the density of mitochrondrial matrix was evident. The in vitro data presented in this report demonstrate the growth inhibitory and antimetabolic effects of mitoxantrone on human breast tumor cells and suggest that the drug may be a promising antitumor agent. of 10-5M. [3H]-thymidine and [ 5 HI-uridine incorporation declined rapidly at the