𝔖 Bobbio Scriptorium
✦   LIBER   ✦

On the role of 17 alpha-estradiol and 17 beta-estradiol in the proliferation of MCF7 and T47D-A11 human breast tumor cells

✍ Scribed by John T. Papendorp; Robert W. Schatz; Ana M. Soto; Carlos Sonnenschein

Publisher
John Wiley and Sons
Year
1985
Tongue
English
Weight
478 KB
Volume
125
Category
Article
ISSN
0021-9541

No coin nor oath required. For personal study only.

✦ Synopsis

A comparative study of the proliferative effect of 17 beta-estradiol and 17 alpha-estradiol on human estrogen-sensitive cell lines was performed. When using charcoal-dextran stripped human female sera-supplemented media the administration of t h e hormones, 17 alpha-estradiol at 3 x IO-"M, and 17 beta-estradiol at 3 x IO-"M, resulted in a ten-fold increase in cell yield when compared with non-estrogen supplemented controls after cells were grown for periods between 10 to 14 days. No significant metabolization of 17 alphaestradiol into 17 beta-estradiol occurred as measured by the E2 levels in the supernatants of the cell culture flasks. Increased concentrations of 17 betaestradiol and 17 alpha-estradiol added to the media bathing C7MCF7-173 cells resulted in a triggering of a partially successful shut-off effect; this phenornenon was not observed with T47D-All cells. These results are compatible with predictions stemming from the indirect and direct negative working hypothesis for t h e regulation of cell Proliferation.

📜 SIMILAR VOLUMES

Characterization of the effects of the n
Characterization of the effects of the novel non-steroidal antiestrogen EM-800 on basal and estrogen-induced proliferation of T-47D, ZR-75-1 and MCF-7 human breast cancer cells in vitro
✍ Jacques Simard; Claude Labrie; Alain Bélanger; Sylvain Gauthier; Shankar M. Sing 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 French ⚖ 256 KB 👁 2 views

Since estrogens play a predominant role in the development and growth of human breast cancer, antiestrogens represent a logical approach to the treatment of this disease. The present study compares the effects of the novel nonsteroidal anti-estrogen EM-800 and related compounds with those of a serie

Evidence on the operation of ATP-induced
Evidence on the operation of ATP-induced capacitative calcium entry in breast cancer cells and its blockade by 17β-estradiol
✍ Ana M. Rossi; Gabriela Picotto; Ana R. de Boland; Ricardo L. Boland 📂 Article 📅 2002 🏛 John Wiley and Sons 🌐 English ⚖ 158 KB

## Abstract Little is known about the regulation of cytosolic calcium Ca^2+^ levels ([Ca^2+^]~i~) in breast cancer cells. We investigated the existence of capacitative calcium entry (CCE) in the tumorigenic cell line MCF‐7 and its responsiveness to ATP. MCF‐7 cells express purinergic receptors as w

Role of PPARγ in the salutary effects of
Role of PPARγ in the salutary effects of 17β-estradiol on kupffer cell cytokine production following trauma-hemorrhage
✍ Takao Suzuki; Takashi Kawasaki; Mashkoor A. Choudhry; Irshad H. Chaudry 📂 Article 📅 2010 🏛 John Wiley and Sons 🌐 English ⚖ 185 KB

## Abstract Studies have shown that administration of 17β‐estradiol prevents trauma‐hemorrhage‐induced increase in proinflammatory cytokine production by Kupffer cells and associated multiple organ injury. Since activation of peroxisome proliferator‐activated receptor γ (PPARγ) following ischemic c