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Influence of methanol on the rotational diffusion of poly(L-lysine) in the helix–coil transition

✍ Scribed by H. Hanssum; H. Rüterjans

Publisher
Wiley (John Wiley & Sons)
Year
1980
Tongue
English
Weight
831 KB
Volume
19
Category
Article
ISSN
0006-3525

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✦ Synopsis

Abstract

^13^C spin‐lattice relaxation times of poly(L‐lysine) have been obtained at 67.9 MHz in aqueous solution and in a mixed solvent (40% methanol/60% water). A concomitant determination of the conformation by CD permits the correlation of conformation and rotational diffusion of the polymer. The dependence on pH of the spin‐lattice relaxation times of the ^13^C^α^ and the side‐chain carbon resonances reflects the diffusional motion in the random‐coil conformation, in the helix–coil transition, and in the conformation of the α‐helix. In the mixed solvent the reorientational correlation time of the C^α^‐H^α^ vector increases from τ = 0.37 nsec (random coil) to τ = 12.0 nsec (α‐helix). In aqueous solution the correlation time of this vector increases from τ = 0.33 nsec (random coil) to τ ≫ 11 nsec. The reorientation rates of the side‐chain methylene groups in the two solvents are markedly different. The reorientation of all methylene groups is reduced in the mixed solvent.

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