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Influence of co-cultures and partial oxygen pressure on DNA and protein content in cultured hepatocytes

✍ Scribed by Holzer, C.; Maier, P.


Book ID
123347454
Publisher
Elsevier Science
Year
1986
Tongue
English
Weight
117 KB
Volume
24
Category
Article
ISSN
0278-6915

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Primary hepatocytes were cultured at oxygen tensions similar to those reported to be present in periportal (13% 02) and pericentral (4% 0,) regions of the liver lobules. Cellular DNA and protein content of individual hepatocytes were determined simultaneously by two-parameter (DNAlprotein) flow cyto