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DNA and protein contents of hepatocytes in primary cultures monitored by flow cytometry: Effect of phenobarbital and dimethylsulphoxide

✍ Scribed by Holzer, C.; Maier, P.


Book ID
122380270
Publisher
Elsevier Science
Year
1987
Tongue
English
Weight
830 KB
Volume
1
Category
Article
ISSN
0887-2333

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Maintenance of periportal and pericentra
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Primary hepatocytes were cultured at oxygen tensions similar to those reported to be present in periportal (13% 02) and pericentral (4% 0,) regions of the liver lobules. Cellular DNA and protein content of individual hepatocytes were determined simultaneously by two-parameter (DNAlprotein) flow cyto