Simultaneous measurements of DNA and protein content of microorganisms by flow cytometry
โ Scribed by K.-J. Hutter; H. E. Eipel
- Book ID
- 104789337
- Publisher
- Springer
- Year
- 1979
- Tongue
- English
- Weight
- 537 KB
- Volume
- 6
- Category
- Article
- ISSN
- 1432-0614
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
Cell division cycle (cdc) mutants of Schizosaccharomyces pombe are arrested at specific points in the cell cycle when grown at restrictive temperature. Flow cytometry of such cells reveals an anomalous increase in the DNA fluorescence signal, which represents a problem in experiments designed to det
Nuclei were isolated from various cell types including Chinese hamster ovary (CHO) and L1210 leukemia cell lines, primary cultures of fibroblasts, nonstimulated and stimulated human lymphocytes and mouse liver cells, by using different isolation techniques. The isolated nuclei were subsequently stai
Background: Analysis of the DNA cell cycle and glutathione content cannot be performed on viable cells, because the fluorescence emissions of the DNA-specific probe Hoechst 33342 and the glutathione-specific probe monobromobimane overlap completely. We decided to explore whether the emissions could