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Induction of somatic embryos in pea,Pisum sativum L.

✍ Scribed by Hans-Jörg Jacobsen; Wilfried Kysely

Publisher
Springer
Year
1984
Tongue
English
Weight
564 KB
Volume
3
Category
Article
ISSN
0167-6857

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✦ Synopsis

Using low concentrations of picloram (0.06 mg/l), embryoids were formed on the surface of leaf-derived callus of pea, Pisum sativum L. (c.v. Dippes Gelbe Victoria) upon transfer to liquid medium. After some days in culture, embryoids spontaneously separated from the calli, and developed into torpedo-shaped embryos, which were transferred to solid medium. In a second series of experiments, embryos were also formed by mutant 489C and a genetic line of Pisum arvense, which additionally exhibited embryogenesis also from epicotyl-derived callus. Some of the embryos showed root formation, but no shoot morphogenesis occurred. In a limited number of cases, an additional root was formed in the apparent shoot apical region after 2-5 days.

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