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Induction of prematurely condensed chromosomes from testicular cells of the mouse

✍ Scribed by Helen L. Drwinga; T. C. Hsu; S. Pathak

Publisher: Springer
Year: 1979
Tongue: English
Weight: 914 KB
Volume: 75
Category: Article
ISSN: 0009-5915
DOI: 10.1007/bf00330623

No coin nor oath required. For personal study only.

✦ Synopsis

Mitotic CHO cells and mouse testicular cells were fused with polyethylene glycol. Several types of prematurely condensed chromosomes were observed. From chromosome morphology it was possible to determine that most of the PCC represented mouse cells. Labeling of either the CHO cells in vitro or the testicular cells in vivo with 3H-TdR prior to fusion also demonstrated that the PCC were derived from the mouse cells. In some PCC, 20 chromosomes could be counted, the haploid number for mouse. It is assumed that these PCC were induced in mouse spermatid nuclei.

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