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Induction of phenoloxidase in cell suspension cultures ofMucuna pruriensL.

✍ Scribed by H. J. Wichers; T. M. Malingré; H. J. Huizing

Publisher
Springer-Verlag
Year
1985
Tongue
English
Weight
495 KB
Volume
165
Category
Article
ISSN
0032-0935

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✦ Synopsis

The effects of limitating nitrogen-containing compounds in the medium and of adding the amino-acid analogues p-fluorophenylalanine and ethionine on both phenoloxidase activity and the accumulation of L-3,4-dihydroxyphenylalanine (L-DOPA) are reported for cell suspension cultures of Mucuna pruriens. Nitrogen limitation of the cultures, or the addition of p-fluorophenylalanine or ethionine to the culture medium resulted in an increased phenoloxidase activity. There appeared to be an inverse relationship between phenoloxidase activity and the acccumulation of L-DOPA. However, the transformation of L-tyrosine into L-DOPA by alginate-entrapped cells occurred at a higher rate when phenoloxidase activity was increased.

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