𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Production of L-DOPA by cell suspension cultures ofMucuna pruriens

✍ Scribed by H. J. Huizing; R. Wijnsma; S. Batterman; Th. M. Malingré; H. J. Wichers

Publisher
Springer
Year
1985
Tongue
English
Weight
564 KB
Volume
4
Category
Article
ISSN
0167-6857

No coin nor oath required. For personal study only.

✦ Synopsis

Methods for induction and maintenance of callus as well as cell suspension cultures of Mucuna pruriens are described. The presence of 3-(3, 4-dihydroxyphenyl 1)-L-alanine (L-DOPA) in these cell suspension cultures was demonstrated by means of TLC and HPLC, the latter being equipped with an ultra-violet or a more specific electrochemical detector. Further methods employed were GC, also in combination with mass spectrometry, and thermal desorption chemical ionization mass spectrometry.

📜 SIMILAR VOLUMES

Production of L-DOPA by cell suspension
Production of L-DOPA by cell suspension cultures ofMucuna pruriens
✍ H. J. Wichers; R. Wijnsma; J. F. Visser; TH. M. Malingré; H. J. Huizing 📂 Article 📅 1985 🏛 Springer 🌐 English ⚖ 356 KB

The endogenous synthesis of 3-(3,4-dihydroxyphenyl)-L-alanine (L-DOPA) by cell suspension cultures of Mucuna pruriens was found to be influenced by several environmental parameters. The nature of the nitrogen source as well as the concentration of nitrogen containing salts, sucrose and phosphate in

Production of L-DOPA from cell suspensio
Production of L-DOPA from cell suspension culture ofMucuna pruriens f. pruriens
✍ Sharmila Chattopadhyay; S. K. Datta; S. B. Mahato 📂 Article 📅 1994 🏛 Springer 🌐 English ⚖ 347 KB

Production of L-DOPA was studied in cell suspension culture of Mucuna pruriens f. pruriens. Suspension culture was established in MSI medium composed of half concentration of Murashige and Skoog's salts and 2% sucrose. A two-stage cell suspension culture was developed for enhanced accumulation of L-

The effect of some environmental factors
The effect of some environmental factors on the production ofL-DOPA by alginate-entrapped cells ofMucuna pruriens
✍ Harm J. Wichers; Theo M. Malingré; Hindrik J. Huizing 📂 Article 📅 1983 🏛 Springer-Verlag 🌐 English ⚖ 445 KB

In-vitro-grown cells of Mucuna pruriens, immobilized in calcium-alginate gels, were able to transform the precursor L-tyrosine into L-dihydroxyphenylalanine (L-DOPA). After the immobilization in alginate the plant cells released 90% of the produced L-DOPA into the medium; supplementation of the medi

Continuous production of the pharmaceuti
Continuous production of the pharmaceutical 7,8-dihydroxy N-di-n-propyl 2-aminotetralin using a phenoloxidase from cell cultures ofMucuna pruriens
✍ Niesko Pras; Sieb Batterman; Durk Dijkstra; Alan S. Horn; Theo M. Malingré 📂 Article 📅 1990 🏛 Springer 🌐 English ⚖ 481 KB

Alginate-entrapped cells of Mucuna pruriens as well as the phenoloxidase isolated from the cell cultures, are able to ortho-hydroxylate several mono-, bi-and tri-cyclic monophenols. In this study, 7,8-dihydroxy N-di-n-propyl 2-aminotetralin, a catechol of pharmaceutical interest and difficult to pre