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Production of L-DOPA by cell suspension cultures ofMucuna pruriens

✍ Scribed by H. J. Wichers; R. Wijnsma; J. F. Visser; TH. M. Malingré; H. J. Huizing

Publisher
Springer
Year
1985
Tongue
English
Weight
356 KB
Volume
4
Category
Article
ISSN
0167-6857

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✦ Synopsis

The endogenous synthesis of 3-(3,4-dihydroxyphenyl)-L-alanine (L-DOPA) by cell suspension cultures of Mucuna pruriens was found to be influenced by several environmental parameters. The nature of the nitrogen source as well as the concentration of nitrogen containing salts, sucrose and phosphate in the culture medium were found to affect the biosynthesis of L-DOPA. Addition of 2, 4-dichlorophenoxyacetic acid to the medium suppressed L-DOPA production; continuous illumination of the cultures had a strong beneficial effect on L-DOPA production. L-DOPA was accumulated intraceUularly by the cell suspension cultures. These observations further demonstrate that for certain products of plant cell suspensions product synthesis can be manipulated by a proper selection of specified nutrients.

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