Induction of mitotic recombination with ethyleneimine in Saccharomyces cerevisiae
β Scribed by F.K. Zimmermann; U. von Laer
- Book ID
- 118952230
- Publisher
- Elsevier Science
- Year
- 1967
- Tongue
- English
- Weight
- 200 KB
- Volume
- 4
- Category
- Article
- ISSN
- 0027-5107
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We have investigated the effects of UV irradiation of Saccharomyces cerevisiae in order to distinguish whether UV-induced recombination results from the induction of enzymes required for homologous recombination, or the production of substrate sites for recombination containing regions of DNA damage
Mitotic cells of a diploid strain of Saccharomyces cerevisiae with appropriate markers for the detection of mitotic crossing-over and mitotic gene conversion were irradiated with X-rays. Induction of these recombinational events was strong. After irradiation, cells were incubated in a rich growth me