Induction of Mitotic Recombination in Saccharomyces cerevisiae by Ethyl Methane Sulphonate
β Scribed by YOST, HENRY T.; CHALEFF, ROY S.; FINERTY, JAMES P.
- Book ID
- 109659318
- Publisher
- Nature Publishing Group
- Year
- 1967
- Tongue
- English
- Weight
- 242 KB
- Volume
- 215
- Category
- Article
- ISSN
- 0028-0836
- DOI
- 10.1038/215660a0
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We have investigated the effects of UV irradiation of Saccharomyces cerevisiae in order to distinguish whether UV-induced recombination results from the induction of enzymes required for homologous recombination, or the production of substrate sites for recombination containing regions of DNA damage
Mitotic cells of a diploid strain of Saccharomyces cerevisiae with appropriate markers for the detection of mitotic crossing-over and mitotic gene conversion were irradiated with X-rays. Induction of these recombinational events was strong. After irradiation, cells were incubated in a rich growth me