Induction of alkaline phosphatase by 5-bromodeoxyuridine in a hybrid line between mouse and Chinese hamster in culture
β Scribed by H. Koyama; T. Ono
- Book ID
- 118875810
- Publisher
- Elsevier Science
- Year
- 1971
- Tongue
- English
- Weight
- 304 KB
- Volume
- 69
- Category
- Article
- ISSN
- 0014-4827
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A clonal hybrid line, B-6, derived from the fusion of mouse and Chinese hamster cell lines, has an inheritable ability to synthesize and secrete hyaluronic acid (HA). In order to elucidate the control mechanism of expression of this differentiated function, we studied the effect of 5-bromodeoxyuridi
## Abstract Nonβlethal concentrations of bromodeoxyuridine induce a 2β to 5βfold increase in the specific activity of alkaline phosphatase in HeLa subclone, S3G. Experiments employing 10βhour pulses of BRdU showed that 48 hours were required before induction commenced, and that maximal induction wa
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