Effect of 5-bromodeoxyuridine on hyaluronic acid synthesis of a clonal hybrid line of mouse and Chinese hamster in culture
✍ Scribed by Hideki Koyama; Tetsuo Ono
- Publisher
- John Wiley and Sons
- Year
- 1971
- Tongue
- English
- Weight
- 546 KB
- Volume
- 78
- Category
- Article
- ISSN
- 0021-9541
No coin nor oath required. For personal study only.
✦ Synopsis
A clonal hybrid line, B-6, derived from the fusion of mouse and Chinese hamster cell lines, has an inheritable ability to synthesize and secrete hyaluronic acid (HA). In order to elucidate the control mechanism of expression of this differentiated function, we studied the effect of 5-bromodeoxyuridine (BUDR), a thymidine (TDR) analog on the growth and HA synthesis of this hybrid line. The presence of BUDR at 0.5-1 sg/ml of medium had no effect on cell multiplication, but reduced HA production by approximately 25 to 40%. Treatment with 5 to 25 pg/ml of the analog resulted in about 15% decrease of the total cell growth, or 30% decrease of the viable cell growth, while mucopolysaccharide production was reduced by more than 70%. These results indicate that BUDR affects HA synthesis more strongly than i t does cell growth.
Inhibition was found to be detectable after two days of culture with BUDR.
This effect was prevented by the simultaneous addition of TDR in a concentration more than twice that of the analog. Furthermore the inhibitory effect was reversible, since a gradual but complete recovery of HA synthesis occurred after removal of BUDR from the medium. In contrast, the growth rate of B-6 cells was discovered to be more sensitive than HA synthesis to the inhibitory action of either excess TDR or another analog, 5-fluorodeoxyuridine (FUDR).