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Induction conditions for maximizing recombinant staphylokinase expression in Escherichia coli

✍ Scribed by Andrés Yarzabal; Marco Bastidas; Luisana Avilan; John Cruz; Juan Puig


Book ID
110369083
Publisher
Springer Netherlands
Year
1997
Tongue
English
Weight
576 KB
Volume
19
Category
Article
ISSN
0141-5492

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We examined the influence of culture conditions on the production of the recombinant fused protein Kringle 1 (Streptokinase) (KI(SK)) expressed in E. co/i under the control of the Ipp-lac promoter. The growth and the protein production were severely inhibited at glucose concentrations higher than 5