Induced Synthesis of O6-Methylguanine-DNA Methyltransferase in Rat Hepatoma Cells Exposed to DNA-damaging Agents
β Scribed by Fukuhara, Masao ;Hayakawa, Hiroshi ;Sakumi, Kunihiko ;Sekiguchi, Mutsuo
- Book ID
- 108579546
- Publisher
- Wiley (Blackwell Publishing)
- Year
- 1992
- Tongue
- English
- Weight
- 374 KB
- Volume
- 83
- Category
- Article
- ISSN
- 0910-5050
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## Abstract The DNA repair protein __O__^6^βmethylguanineβDNA methyltransferase (MGMT) has been implicated in resistance of human brain tumors to alkylating agents. We observed that 14 human medulloblastomaβ and gliomaderived cell lines differ in sensitivity to the methylating agent __N__βmethylβ__
We have constructed a plasmid in which the expression of human O6-methylguanine-DNA methyltransferase (MGMT) cDNA is driven by the Rous sarcoma virus promoter sequence. Transfection of this plasmid into Chinese hamster ovary (CHO) cells results in expression of MGMT and in cellular resistance to N-m