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Individual and simultaneous enzymatic determination of ethanol and acetaldehyde in wines by flow injection analysis

✍ Scribed by F. Lázaro; M.D.Luque de Castro; M. Valcárcel

Publisher
Elsevier Science
Year
1986
Tongue
English
Weight
470 KB
Volume
185
Category
Article
ISSN
0003-2670

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✦ Synopsis

The individual and simultaneous enzymatic determination of ethanol and acetaldehyde in wine by flow injection analysis is described. Individual determinations of 0.002-0.016% (v/v) ethanol or 1.0-8.0 rg ml-' acetaldehyde with r.s.d. 0.7% and 0.5%, respectively, are done with a single-beam spectrophotometer, based on the use of alcohol dehydrogenase and aldehyde dehydrogenase. A diode-array detector and dual reagent injections are used for the simultaneous determination of the two compounds. The errors are <3.5% and <2.0% for ethanol and acetaldehyde, respectively, when the method is applied to wine samples.

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