Independent regulation of prostaglandin production and the stress response in human fibroblasts

✍ Scribed by J. Salzman; P. D. Bowman

• Publisher: John Wiley and Sons
• Year: 1992
• Tongue: English
• Weight: 579 KB
• Volume: 152
• Category: Article
• ISSN: 0021-9541
• DOI: 10.1002/jcp.1041520322

✦ Synopsis

FrdnCiSCo, and letterman Army I n M u t e of Recearch, Presidio of S a n Francisco, California 94 129-6800

The stress, or heat shock response of eukaryotic cells is characterized by dramatic changes in the metabolism of respondingcells, most notably the increased synthesis o f a group of proteins known as heat shock proteins. In this study, we examined the relationship of prostaglandin synthcsisireleasc to the stress response. Stress protein synthesis was induced with sodium arsenite, and prostaglandin E, and prostdcyclin (measured as 6-keto PGF,,) levels were determined by enzyme immunoassay. Thc stress response was monitored by the incorporation of [15S]methionine and separation of protein by one-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Prostaglandin synthesis and thc strcss response were both induced by sodium arsenite. However, aspirin, a cyclooxygenase inhibitor, inhibited arsenite-induced prostaglandin synthesis but did not inhibit stress protein synthesis. Conversely, the calcium ionophore A2.5 187 also stimulated prostaglandin synthesis, but did not induce the stress response. The results of this study indicate that sodium arsenite, stre55 response inducer, stimulates prostaglandin production, but this appears to be a correlative rather than causative occurrence in the strcss response. Determirialion of the cytotoxicity of arscnitc indicated a high correlation of stimulation of prostaglandin release with cytotoxicity.