Increase of embryogenic callus formation in cucumber by initial culture on high concentration of 2,4-dichlorophenoxyacetic acid

✍ Scribed by Anne-Marie Kuijpers; Han Bouman; Geert-Jan Klerk

• Book ID: 104618198
• Publisher: Springer
• Year: 1996
• Tongue: English
• Weight: 191 KB
• Volume: 46
• Category: Article
• ISSN: 0167-6857
• DOI: 10.1007/bf00039699

✦ Synopsis

Cucumber (Cucumis sativus L.) leaf explants were cultured either continuously on standard medium containing 4.5 #M 2,4-dichlorophenoxyacetic acid (2,4-D) and 4.4 #M benzylaminopurine, or first cultured for various periods at different levels of 2,4-D, picloram or naphthaleneacetic acid (NAA), and then transferred to standard medium. When cultured continuously on standard medium, less than 10% of the explants formed embryogenic callus. Initial culture on picloram or NAA, or on 2,4-D at a low concentration (1.4 #M) did not result in any embryogenic callus formation. Embryogenic callus formation increased to 40% if during the initial phase of the culture (10 days), the 2,4-D concentration was raised to 14 #M. Prolonged culture on 14 #M 2,4-D resulted in less embryogenic callus formation.