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Inclusion body purification and protein refolding using microfiltration and size exclusion chromatography

✍ Scribed by Boris Batas; Chiara Schiraldi; Julian B. Chaudhuri

Book ID
118045832
Publisher
Elsevier Science
Year
1999
Tongue
English
Weight
201 KB
Volume
68
Category
Article
ISSN
0168-1656

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Protein refolding at high concentration
Protein refolding at high concentration using size-exclusion chromatography
✍ Boris Batas; Julian B. Chaudhuri πŸ“‚ Article πŸ“… 1996 πŸ› John Wiley and Sons 🌐 English βš– 809 KB

A new method to improve refolding yields and t o increase the concentration of refolded proteins in a single operation has been developed. The method uses size-exclusion chromatography matrices to perform buffer exchange, aggregate removal, and the folding reaction. The reduced diffusion of proteins