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Refolding and purification of recombinant human interferon-γ expressed as inclusion bodies inEscherichia coliusing size exclusion chromatography

✍ Scribed by Yi-Xin Guan; Hai-Xue Pan; Yong-Gui Gao; Shan-Jing Yao; Man-Gi Cho


Publisher
The Korean Society for Biotechnology and Bioengineering
Year
2005
Tongue
English
Weight
409 KB
Volume
10
Category
Article
ISSN
1226-8372

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On-column refolding of recombinant human
✍ Ting Jin; Yi-Xin Guan; Shan-Jing Yao; Dong-Qiang Lin; Man-Gi Cho 📂 Article 📅 2006 🏛 John Wiley and Sons 🌐 English ⚖ 133 KB 👁 1 views

A refolding strategy was described for oncolumn refolding of recombinant human interferong (rhIFN-g) inclusion bodies by expanded bed adsorption (EBA) chromatography. After the denatured rhIFN-g protein bound onto the cation exchanger of STREAMLINE SP, the refolding process was performed in expanded