𝔖 Bobbio Scriptorium
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Inactivation of the α-actinin gene inDictyostelium

✍ Scribed by Noegel, Angelika A. ;Witke, Walter

Publisher
John Wiley and Sons
Year
1988
Tongue
English
Weight
433 KB
Volume
9
Category
Article
ISSN
0192-253X

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✦ Synopsis

a-Actinin-negative transformants of Dicryostelium have been obtained by transforming cells with a transformation vector carrying part of the a-actinin gene in either sense or antisense orientation. The transformants did not produce detectable a-actinin anymore and contained an altered RNA lacking the 3' part of the coding sequences. The deficiency in a-actinin was due to an integration of the transformation vector into the gene, since it could be detected by Southern blot analysis in the endogenous gene.

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