In vivo transfer of genes fromEscherichia colitoBacillus subtilis
✍ Scribed by G. Buheitel; W. Klingmüller
- Publisher
- Springer
- Year
- 1979
- Tongue
- English
- Weight
- 310 KB
- Volume
- 7
- Category
- Article
- ISSN
- 1432-0614
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around 7% of chimeric clones assuming that an average full-length ds cDNA contains six RsaI sites (1 of 14; total of 14 ends in seven cDNA fragments). Unlike the 5Ј end cDNA fragment, the 3Ј end fragment has two RsaI blunt ends because the cDNA synthesis primer used in SSH contains a RsaI site. In