In vivo myocardial gene transfer: Optimization, evaluation and direct comparison of gene transfer vectors
โ Scribed by Matt J. Wright; Lionel M. L. Wightman; Caroline Lilley; Mahesh de Alwis; Steven L. Hart; Andrew Miller; Robert S. Coffin; Adrian Thrasher; David S. Latchman; Michael S. Marber
- Publisher
- Springer
- Year
- 2001
- Tongue
- English
- Weight
- 541 KB
- Volume
- 96
- Category
- Article
- ISSN
- 0300-8428
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## Abstract ## Background Because gene therapy of the future will primarily take an __in vivo__ approach, a number of problems associated with its current implementation exist. Currently, repeated delivery of a vector __in vivo__ is necessary to ensure adequate transfer of the therapeutic gene. Th
Bacterial โค-galactosidase cDNA was injected without lipofectin into 41 sites in dog muscle and expression was seen in 22 of them. The cDNA and lipofectin was injected into 35 similar sites and expression was seen in 21. Expression was seen in a maximum of 2.5% of muscle fibers and 23.21% of nonmuscl