๐”– Bobbio Scriptorium
โœฆ   LIBER   โœฆ

In vivo synthesis and purification of radioactive p-hydroxybenzylglucosinolate in Sinapis alba L.

โœ Scribed by Sixue Chen; Barbara Ann Halkier

Publisher
John Wiley and Sons
Year
2000
Tongue
English
Weight
156 KB
Volume
11
Category
Article
ISSN
0958-0344

No coin nor oath required. For personal study only.

โœฆ Synopsis

Glucosinolates are amino acid-derived, natural plant products found in the order Capparales. Radiolabelled glucosinolates for use in biological studies concerning metabolism and transport are not commercially available. We have developed a method for the synthesis and isolation of radiolabelled phydroxybenzylglucosinolate (p-OHBG) produced in vivo by Sinapis alba, which has the tyrosine-derived p-OHBG as its major glucosinolate. The method is based on administration of radiolabelled tyrosine to jasmonate-treated primary leaves, which have high de novo biosynthesis of p-OHBG. After incubation, the plant material was extracted with methanol and the glucosinolates purified by a procedure including two ion exchange chromatographic steps. The method provides an attractive alternative to chemical synthesis of radiolabelled glucosinolates and is applicable to other plants producing a single or one major glucosinolate.

๐Ÿ“œ SIMILAR VOLUMES

Preparation and purification of 77Br-lab
Preparation and purification of 77Br-labelled p-bromospiroperidol suitable for in vivo dopamine receptor studies
โœ Onofre T. Dejesus; Arnold M. Friedman; Anita Prasad; James R. Revenaugh ๐Ÿ“‚ Article ๐Ÿ“… 1983 ๐Ÿ› John Wiley and Sons ๐ŸŒ French โš– 389 KB

An e f f i c i e n t and r e l i a b l e method of p r e p a r i n g 77Br-p-bromo s p i r o p e r i d o l s u i t a b l e f o r l a b e l l i n g dopamine r e c e p t o r s i n v i v o h s been developed. T h i s s y n t h e s i s i n v o l v e s t h e r e a c t i o n of 7'Br-, H202, and s p i r o p

New possibilities of enzymic synthesis o
New possibilities of enzymic synthesis of radioactive nucleotides. IV. Application of in vivo tryptophan-stabilized enzyme system from regenerating rat liver for the synthesis of radioactive thymidine 5โ€ฒ - phosphates in vitro
โœ Jiri Filip; Karel Hybs; Zdenek Nejedly; Januaz Greger; Jiri Vesely; Alois Cihak ๐Ÿ“‚ Article ๐Ÿ“… 1972 ๐Ÿ› John Wiley and Sons ๐ŸŒ French โš– 313 KB

The e n z y m a t i c e x t r a c t p t e p a r e d f r o m 48h. r e g e n e r at i n g r a t l i v e r s t a b i l i z e d w i t h d i e t a r y L -t r y p t o p h a n p o s s e s s e s t h e enhanced t h y m i d i n e and t h y m i d y l a t e k inaae a c t i v i t y . The o p t i m a l c o n d i